Southern and Northern blotting methods from "summary" of Molecular Cloning by Joseph Sambrook,David William Russell
Southern and Northern blotting methods are widely used for the analysis of DNA and RNA molecules, respectively. Southern blotting allows for the detection of specific DNA sequences in a complex mixture, while Northern blotting is used to detect specific RNA molecules. These methods have proven to be invaluable in molecular biology research for the study of gene expression and genetic variation. In Southern blotting, DNA fragments are first separated by gel electrophoresis, usually on an agarose gel. The DNA is then denatured and transferred to a solid support, such as a nitrocellulose or nylon membrane. The DNA on the membrane is then exposed to a labeled DNA probe that is complementary to the target sequence. The probe will hybridize to the target DNA, allowing for its detection by autoradiography or other detection meth...
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