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Molecular Cloning: A Laboratory Manual is a comprehensive guide to the techniques and principles of cloning. Written by four experts in the field, the book covers the fundamentals of recombinant DNA technology, from the basics of nucleic acid chemistry to the most advanced cloning methods. It also provides extensive guidance on the various laboratory methods and procedures involved in cloning, including the isolation of DNA, the construction of vectors, the transformation of cells, and the expression of cloned genes. The book also includes detailed protocols for working with cloning systems in a variety of organisms, including Escherichia coli, Saccharomyces cerevisiae, and mammalian cells. Molecular Cloning is a valuable resource for students, researchers, and professionals in the field of recombinant DNA technology.
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