Importance of restriction enzymes from "summary" of Molecular Cloning by Joseph Sambrook,David William Russell
Restriction enzymes play a critical role in molecular biology by cleaving DNA at specific recognition sequences. These enzymes are essential tools for manipulating DNA in a controlled and precise manner. They are commonly used in various molecular cloning techniques, such as gene cloning, gene mapping, and DNA fingerprinting. The specificity of restriction enzymes allows researchers to target specific DNA sequences for manipulation. By cutting DNA at precise locations, scientists can create DNA fragments with known sequences. These fragments can then be ligated together to create recombinant DNA molecules. This ability to cut and paste DNA sequences is fundamental to many molecular biology experiments. Researchers can use restriction enzymes to insert foreign DNA into a host organism's genome. By cutting both the foreign DNA and the host DNA with the same restriction enzyme, scientists can create compatible ends that...
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