Polymerase chain reaction (PCR) applications from "summary" of Molecular Cloning by Joseph Sambrook,David William Russell
Polymerase chain reaction (PCR) is a powerful tool that has revolutionized molecular biology research. The applications of PCR are vast and diverse, making it an indispensable technique in virtually every aspect of biological research. One of the most common applications of PCR is in the amplification of specific DNA sequences. By using specific primers that flank the region of interest, PCR can selectively amplify the desired DNA fragment from a complex mixture of genomic DNA. PCR is also widely used in the cloning of DNA fragments. Once a DNA fragment has been amplified by PCR, it can be easily cloned into a vector for further manipulation. This allows researchers to study the function of the gene encoded by the DNA fragment, as well as to produce large quantitie...
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