Basics of DNA manipulation techniques from "summary" of Molecular Cloning by Joseph Sambrook,David William Russell
Understanding the basics of DNA manipulation techniques is essential for any researcher working in the field of molecular biology. These techniques involve the manipulation of DNA molecules in order to study their structure, function, and interactions. By mastering these techniques, researchers can isolate specific DNA sequences, clone genes, and analyze gene expression. One of the fundamental techniques in DNA manipulation is the polymerase chain reaction (PCR), which allows for the amplification of specific DNA sequences. PCR involves cycles of denaturation, annealing, and extension, resulting in the exponential amplification of the target DNA. This technique is widely used in molecular biology for various applications, such as gene cloning, sequencing, and genetic testing. Another important technique in DNA manipulation i...
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