Gene expression studies from "summary" of Molecular Cloning by Joseph Sambrook,David William Russell
Gene expression studies involve the analysis of the processes by which the genetic information encoded in a gene is used to direct the synthesis of a functional gene product. This can include the transcription of the gene into RNA, the processing of RNA, and the translation of RNA into protein. Gene expression studies are crucial for understanding how genes are regulated and how changes in gene expression can lead to different phenotypes or diseases. Researchers use a variety of techniques to study gene expression, including Northern blotting, reverse transcription polymerase chain reaction (RT-PCR), in situ hybridization, and microarray analysis. These techniques allow researchers to measure the levels of mRNA for a particular gene in different tissues or under different conditions. By comparing gene expression profiles between normal and diseased tissues...
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