Understanding plasmids and vectors from "summary" of Molecular Cloning by Joseph Sambrook,David William Russell
Plasmids are small, circular DNA molecules that are capable of replicating independently of the chromosomal DNA in a bacterial cell. These extrachromosomal elements have been extensively studied and manipulated for use in molecular cloning experiments. Plasmids can carry foreign DNA fragments inserted into their sequences, making them valuable tools for the construction and propagation of recombinant DNA molecules. Vectors, on the other hand, are vehicles used to transfer foreign DNA into a host cell. Plasmids are a common type of vector used in molecular cloning due to their ability to replicate autonomously and carry foreign DNA sequences. Vectors typically contain features such as origin of replication, selectable markers, and cloning sites to facilitate the introduction an...
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