Selecting transformed cells from "summary" of Molecular Cloning by Joseph Sambrook,David William Russell
After the introduction of the foreign DNA into bacterial cells, the next crucial step in molecular cloning is the selection of cells that have taken up the desired DNA. This selection process is essential to identify and isolate the transformed cells that contain the recombinant DNA of interest. Without proper selection, it would be nearly impossible to distinguish between transformed and non-transformed cells, making the isolation of the desired DNA a challenging task.
To achieve this selection, scientists often use a technique known as antibiotic selection. In this method, the foreign DNA is usually introduced into the bacterial cells along with a gene that confers resistance to a specific antibiotic. This antibiotic resistance gene is typically located within the same vector as the foreign DNA, allowing for both the insertion of the desired DNA and the selection of transformed cells in a single step.
After the transformation process, the bacterial cells are p...
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