RNA interference (RNAi) applications from "summary" of Molecular Cloning by Joseph Sambrook,David William Russell
RNA interference (RNAi) applications have revolutionized the field of molecular biology by providing a powerful tool for gene silencing. This technology utilizes small RNA molecules to target specific messenger RNAs (mRNAs) for degradation, ultimately leading to the suppression of gene expression. One of the key advantages of RNAi is its ability to knock down gene expression with high specificity, allowing researchers to investigate the function of individual genes in a targeted manner. The process of RNA interference involves the introduction of small interfering RNAs (siRNAs) or short hairpin RNAs (shRNAs) into cells, which are then processed by the cellular machinery into small RNA molecules that guide the RNA-induced silencing complex (RISC) to complementary mRNAs. Once bound to the target mRNA, the RISC complex cleaves the mRNA, leading to its degradation and the subsequent reduction in protein levels. This mechanism can be exploited to silence genes of interest in a wide range of biological systems, from cell culture models to whole organ...
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