Realtime PCR for quantitative analysis from "summary" of Molecular Cloning by Joseph Sambrook,David William Russell
Real-time PCR is a powerful technique for quantitative analysis of nucleic acids. It is based on the polymerase chain reaction, in which a specific DNA sequence is amplified exponentially. However, in real-time PCR, the amplification of DNA is monitored in "real time" as the reaction is taking place, allowing for the quantification of the starting amount of DNA. One of the key components of real-time PCR is the use of fluorescent dyes or probes that bind specifically to the amplified DNA. These dyes emit a fluorescent signal that can be detected by a thermal cycler in each cycle of the PCR reaction. The amount of fluorescence is directly proportional ...
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