Introduction to molecular cloning from "summary" of Molecular Cloning by Joseph Sambrook,David William Russell
The process of molecular cloning involves the isolation and replication of a specific DNA sequence in order to study its structure, function, or expression. This technique has revolutionized the field of molecular biology by allowing scientists to manipulate and analyze genes with unprecedented precision. One of the first steps in molecular cloning is the isolation of the DNA fragment of interest, which can be achieved using a variety of methods such as polymerase chain reaction (PCR), restriction enzyme digestion, or genomic library screening. Once the DNA fragment has been isolated, it must be inserted into a vector - a small, circular piece of DNA that can replicate independently in a host cell. Vectors serve as vehicles for int...
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