Gene knockout strategies from "summary" of Molecular Cloning by Joseph Sambrook,David William Russell
Gene knockout strategies involve the targeted disruption of a specific gene to investigate its function. This can be achieved by introducing mutations into the gene, resulting in its inactivation. One common method for gene knockout is the use of homologous recombination to replace the wild-type gene with a mutated version. This approach allows for precise control over the location and nature of the mutation, resulting in the specific disruption of the gene of interest. Another strategy for gene knockout involves the use of RNA interference (RNAi) to silence gene expression. This technique utilizes small interfering RNAs (siRNAs) to target and degrade...
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