DNA sequencing techniques from "summary" of Molecular Cloning by Joseph Sambrook,David William Russell
DNA sequencing techniques are essential tools in modern molecular biology research. These methods allow scientists to decipher the genetic information encoded in DNA molecules with unprecedented accuracy and efficiency. As a result, DNA sequencing has become a cornerstone of many scientific disciplines, from genetics and genomics to evolutionary biology and personalized medicine. One of the earliest techniques developed for DNA sequencing is the Sanger method, named after its inventor Frederick Sanger. This method relies on the enzymatic synthesis of DNA molecules in the presence of chain-terminating nucleotides, which are labeled with fluorescent dyes. The resulting DNA fragments are separated by size using gel electrophoresis, allowing researchers to read the sequence of nucleotides in the original DNA molecule. More recent advances in DNA sequencing technology have led to the development of high-throughput sequencing platforms, also known as next-generation sequencing (NGS) technologies. These platforms ...
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