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Deanna Glover

Sample Preparation for UV-VIS Spectroscopy

1. Sample preparation for UV-VIS spectroscopy is an important step for successful analysis. 2. Make sure to use a suitable sample solvent for the analysis and size the sample to the desired concentration. 3. Avoid contaminants as these can affect the accuracy of the results. 4. Filter the sample solution and degas it if necessary. 5. Check the absorbance of the sample at different wavelengths so that you can determine the optimal wavelength to use for analysis. 6. Make sure to record the dilution factors used to prepare the sample solution. 7. Monitor the pH and temperature of the sample solution during the experiment. 8. Use quality control samples to ensure the accuracy of the results.

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UV-VIS Spectroscopy and Its Applications

Heinz-Helmut Perkampus

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