Chromatographic methods in drug analysis from "summary" of Chemistry for Pharmacy Students by Lutfun Nahar,Professor Satyajit D. Sarker
Chromatographic methods are widely used in drug analysis to separate and quantify the components of complex mixtures. These methods are based on the principle of differential partitioning of the components between a stationary phase and a mobile phase. The stationary phase can be a solid or liquid material, while the mobile phase is typically a liquid or gas. One of the most commonly used chromatographic methods in drug analysis is high-performance liquid chromatography (HPLC). In HPLC, the sample mixture is injected into a column packed with a stationary phase, and a solvent is pumped through the column at high pressure. The components in the sample interact differently with the stationary phase, leading to their separation as they pass through the column. Detection of the separated components is typically done using ultraviolet (UV) or fluorescence detectors. Another chromatographic method used ...
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