Chromatographic methods in drug analysis from "summary" of Chemistry for Pharmacy Students by Lutfun Nahar,Professor Satyajit D. Sarker
Chromatographic methods are widely used in drug analysis to separate and quantify the components of complex mixtures. These methods are based on the principle of differential partitioning of the components between a stationary phase and a mobile phase. The stationary phase can be a solid or liquid material, while the mobile phase is typically a liquid or gas. One of the most commonly used chromatographic methods in drug analysis is high-performance liquid chromatography (HPLC). In HPLC, the sample mixture is injected into a column packed with a stationary phase, and a solvent is pumped through the column at high pressure. The components in the sample interact differently with the stationary phase, leading to their separation as they pass through the column. Detection of the separated components is typically done using ultraviolet (UV) or fluorescence detectors. Another chromatographic method used in drug analysis is gas chromatography (GC). In GC, the sample is vaporized and injected into a column packed with a stationary phase, which is typically a coated capillary column. The sample components are separated as they interact with the stationary phase and the carrier gas flowing through the column. Detection of the separated components is usually done using a mass spectrometer. Thin-layer chromatography (TLC) is another chromatographic method that is commonly used in drug analysis. In TLC, the sample mixture is spotted onto a thin layer of adsorbent material on a plate. The plate is then placed in a solvent chamber, and the components in the sample mixture move up the plate at different rates based on their interaction with the adsorbent material. Detection of the separated components is typically done by visualizing the spots under ultraviolet light or by using a suitable chemical reagent.- Chromatographic methods play a crucial role in drug analysis by allowing for the separation and quantification of the components in complex mixtures. HPLC, GC, and TLC are some of the commonly used chromatographic methods in drug analysis, each offering unique advantages and applications in pharmaceutical analysis.
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